Journal of Forensic and Legal Medicine
Volume 15, Issue 6 , Pages 368-372 , August 2008

Immunohistochemical analysis of P-Selectin as a possible marker of vitality in human cutaneous wounds

  • J.A. Ortiz-Rey, MD

      Affiliations

    • Department of Pathology, POVISA Hospital, Vigo, Spain
    • Corresponding Author InformationCorresponding author. Address: Servicio de Anatomía Patológica, Hospital POVISA, Salamanca 5, 36211 Vigo, Spain. Tel.: +34 986 413 144; fax: +34 986 421 439.
    • ,
  • J.M. Suárez-Peñaranda, MD PhD

      Affiliations

    • Department of Pathology and Forensic Sciences, University of Santiago de Compostela, Spain
    • ,
  • P. San Miguel, MD PhD

      Affiliations

    • Department of Pathology, POVISA Hospital, Vigo, Spain
    • ,
  • J.I. Muñoz, MD PhD

      Affiliations

    • Department of Pathology and Forensic Sciences, University of Santiago de Compostela, Spain
    • ,
  • M.S. Rodríguez-Calvo, MD PhD

      Affiliations

    • Department of Pathology and Forensic Sciences, University of Santiago de Compostela, Spain
    • ,
  • L. Concheiro, MD PhD

      Affiliations

    • Department of Pathology and Forensic Sciences, University of Santiago de Compostela, Spain

Received 22 November 2007 ,Revised 15 January 2008 ,Accepted 8 February 2008.

References 

  1. Betz P. Histological and enzyme histochemical parameters for the age estimation of human skin wounds. Int J Legal Med. 1994;107:60–68
  2. Ohshima T. Forensic wound examination. Forensic Sci Int. 2000;113:153–164
  3. Madea B. Importance of supravitality in forensic medicine. Forensic Sci Int. 1994;69:221–241
  4. Viljanto J, Penttinen R, Raekallio J. Fibronectin in early phases of wound healing in children. Acta Clin Scand. 1981;47:7–13
  5. Betz P, Nerlich A, Wilske J, Tübel J, Wiest I, Penning R, et al. Immunohistochemical localisation of fibronectin as a tool for the age determination of human skin wounds. Int J Legal Med. 1992;105:21–26
  6. Betz P. Immunohistochemical parameters for the age estimation of human skin wounds: a review. Am J Forensic Med Pathol. 1995;16:203–209
  7. Dressler J, Bachmann L, Koch R, Müller E. Enhanced expression of selectins in human skin wounds. Int J Legal Med. 1998;112:39–44
  8. Grellner W, Dimmeler S, Madea B. Immunohistochemical detection of fibronectin in postmortem incised wounds of porcine skin. Forensic Sci Int. 1998;97:109–116
  9. Ortiz-Rey JA, Suárez-Peñaranda JM, Da Silva EA, Muñoz JI, San Miguel-Fraile P, De la Fuente-Buceta, et al. Immunohistochemical detection of fibronectin and tenascin in incised human skin injuries. Forensic Sci Int. 2002;126:118–122
  10. Ortiz-Rey JA, Suárez-Peñaranda JM, Muñoz-Barús JI, Álvarez C, San Miguel P, Rodríguez Calvo MS, et al. Expression of fibronectin and tenascin as a demonstration of vital reaction in rat skin and muscle. Int J Legal Med. 2003;117:356–360
  11. McEver RP, Martin MN. A monoclonal antibody to a membrane glycoprotein binds only to activated platelets. J Biol Chem. 1984;259:9799–9804
  12. Stenberg PE, McEver RP, Shuman MA, Jacques YV, Bainton DF. A platelet alpha-granule membrane protein (GMP-140) is expressed on the plasma membrane after activation. J Cell Biol. 1985;101:880–886
  13. Johnston GI, Cook RG, McEver RP. Cloning of GMP-140, a granule membrane protein of platelets and endothelium: sequence similarity to proteins involved in cell adhesion and inflammation. Cell. 1989;56:1033–1044
  14. McEver RP, Moore KL, Cummings RD. Leukocyte trafficking mediated by selectin-carbohydrate interactions. J Biol Chem. 1995;270:11025–11028
  15. Lorant DE, Topham MK, Whatley RE, McEver RP, McIntyre TM, Prescott SM, et al. Inflammatory roles of P-Selectin. J Clin Invest. 1993;92:559–570
  16. Dressler J, Bachmann L, Strejc P, Koch R, Müller E. Expression of adhesion molecules in skin wounds: diagnostic value in legal medicine. Forensic Sci Int. 2000;113:173–176
  17. Grellner W, Madea B. Demands on scientific studies: vitality of wounds and wound age estimation. Forensic Sci Int. 2007;165:150–154
  18. Hernández Cueto C. Diagnóstico diferencial entre lesiones vitales y postmortales. In:  Villanueva Cañadas E editors. Medicina legal y toxicología Gisbert Calabuig. 6th ed.. Barcelona: Masson; 2004;p. 351–359
  19. Rosen SD. Selectins. In:  Kreis T,  Vale R editor. Guidebook to the extracellular matrix and adhesion proteins. 1st ed.. New York: Oxford University Press; 1993;p. 168–171
  20. Ortmann C, Brinkmann B. The expression of P-Selectin in inflammatory and non-inflammatory lung tissue. Int J Legal Med. 1997;110:155–158
  21. Tsokos M, Fehlauer F, Püschel K. Immunohistochemical expression of E-Selectin in sepsis-induced lung injury. Int J Legal Med. 2000;113:338–342
  22. Ali TT. The role of white blood cells in post-mortem wounds. Med Sci Law. 1988;28:100–106
  23. Grellner W, Madea B, Kruppenbacher JP, Dimmeler S. Interleukin-1α (IL-1α) and N-formyl-methionyl-leucyl-phenylalanine (FMLP) as potential inducers of supravital chemotaxis. Int J Legal Med. 1996;109:130–133
  24. Pelstring RJ, Allred DC, Esther RJ, Lampkin SR, Banks PM. Differential antigen preservation during tissue autolysis. Hum Pathol. 1991;22:237–241
  25. Fieguth A, Kleemann WJ, von Wasielewski R, Werner M, Tröger HD. Influence of postmortem changes on immunohistochemical reactions in skin. Int J Legal Med. 1997;110:18–21

☆ Supported in part by Grant PGIDT01PXI20807PR.

☆☆ Presented in part at the 17th Meeting of the International Association of Forensic Sciences, Hong Kong, August 21–26, 2005.

PII: S1752-928X(08)00028-0

doi: 10.1016/j.jflm.2008.02.011

Journal of Forensic and Legal Medicine
Volume 15, Issue 6 , Pages 368-372 , August 2008

Article Tools

* Image Usage & Resolution